Rapid quantification of prion proteins using resistive pulse sensing

文献情報

出版日 2020-02-12

DOI 10.1039/D0AN00063A

インパクトファクター 4.616

著者

Matthew J. Healey, Muttuswamy Sivakumaran, Mark Platt

原文を見る

要旨

Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of intrinsic cellular prion protein (PrPC). We present a rapid assay using aptamers and resistive pulse sensing, RPS, to extract and quantify PrPC from complex sample matrices. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB's termed P-beads, are used to pre-concentrate the analyte from a large sample volume. The PrPC protein is then eluted from the P-beads before aptamer modified sensing beads, S-beads, are added. The velocity of the S-beads through the nanopore reveals the concentration of the PrPC protein. The process is done in under an hour and allows the detection of picomol's of protein.

掲載誌

Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Rapid quantification of prion proteins using resistive pulse sensing

文献情報

おすすめジャーナル

関連文献

Growth of aligned SWNT arrays from water-soluble molecular clusters for nanotube device fabrication

Resonance Raman effects in TS-1: the structure of Ti(iv) species and reactivity towards H2O, NH3 and H2O2: an in situ study

A kinetic study of the reactions of Fe+ with N2O, N2, O2, CO2 and H2O, and the ligand-switching reactions Fe+·X + Y → Fe+·Y + X (X = N2, O2, CO2; Y = O2, H2O)

Back cover

Thermophoresis as a probe of particle–solvent interactions: The case of protein solutions

Unconventional vesicle-to-ribbon transition behaviour of diacyl glycerol amino acid based surfactants in extremely diluted systems induced by pH-concentration effects

The cathodic behaviour of poly(alkyl) and (aryl) sulfones revisited. Mechanistic and theoretical approaches

Elucidation of the local structure of active titanium(iv) sites on silica-based phase-boundary catalysts for alkeneepoxidation with aqueous hydrogen peroxide

Ring inversion kinetics of p-sulfonatocalix[4]arene and of its Ca(ii) and La(iii) complexes in water and water-acetone solutions

Using real-valued multi-objective genetic algorithms to model molecular absorption spectra and Raman excitation profiles in solution

こちらもおすすめ

N,N-二乙基-4-ブロモナフサルレン-1-カルボニルアミドはどのように合成されますか？

大黄酚-8-O-葡萄糖苷の市場動向や研究トレンドはどうですか？

アトラキュリウム不純物5塩酸塩の物理化学的性質は何ですか？

2-イソブチルシクロヘキサン酮とは何ですか？

2-溴-6-甲基烟酸を取り扱う際の実験室安全事項は何ですか？

6-アミノニコニタルデオキシド塩化水和物の物理化学的性質は何ですか？

塩酸中毒藜碱はどのように合成されますか？

Methyl 4-(6-formyl-2-pyridinyl)benzoateに適用される法規ガイドラインは何ですか？

1-エチynyル-3-(三氟甲氧基)ベンゼンについて「に適用される法規ガイドラインは何ですか」

メチル2-ブロモイソニコネートの代替品はありますか？

掲載誌

Analyst

おすすめ化合物

1-Benzyl-3-pyrrolidinol

(6-Chloro-3-pyridinyl)methanol

3-Hydroxy-2-oxo-3-phenylpropanoic acid

Ethyl 2-chloronicotinate

Adenosine 5'-Triphosphate as disodium salt

おすすめサプライヤー