Facile analysis of metabolites by capillary electrophoresis coupled to matrix-assisted laser desorption/ionization mass spectrometry using target plates with polysilazane nanocoating and grooves

文献情報

出版日 2009-05-27

DOI 10.1039/B907039G

インパクトファクター 4.616

著者

Andrea Amantonico, Pawel L. Urban, Renato Zenobi

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要旨

We present an inexpensive method and apparatus for the deposition of analytes separated by capillary electrophoresis (CE) onto a custom-designed matrix-assisted laser desorption/ionization (MALDI) target plate. This dedicated CE-MALDI plate is coated with an omniphobic polysilazane nanocoating and has an array of parallel grooves acting as recipients of the separation effluent. The 3-D pattern in the top layer of the coated plate greatly improves loading of the matrix solution prior to separation and facilitates deposition of the separated species. We demonstrate application of this straightforward protocol to the analysis of metabolites from the central metabolic pathway in a complex biological sample spiked with small molecule standards.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

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Facile analysis of metabolites by capillary electrophoresis coupled to matrix-assisted laser desorption/ionization mass spectrometry using target plates with polysilazane nanocoating and grooves

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