Twist–stretch coupling and phase transition during DNA supercoiling

文献情報

出版日 2009-05-14

DOI 10.1039/B901646E

インパクトファクター 3.676

著者

Maxim Y. Sheinin

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要旨

As a single DNA molecule is positively supercoiled under constant tension, its extension initially increases due to a negative twist–stretch coupling. The subsequent attainment of an extension maximum has previously been assumed to be indicative of the onset of a phase transition from B- to scP-DNA. Here we show that an extension maximum in fact does not coincide with the onset of a phase transition. This transition is evidenced by a direct observation of a torque plateau using an angular optical trap. Instead we find that the shape of the extension curve can be well explained with a theory that incorporates both DNA twist–stretch coupling and bending fluctuations. This theory also provides a more accurate method of determining the value of the twist–stretch coupling modulus, which has possibly been underestimated in previous studies that did not take into consideration the bending fluctuations. Our study demonstrates the importance of torque detection in the correct identification of phase transitions as well as the contribution of the twist–stretch coupling and bending fluctuations to DNA extension.

掲載誌

Physical Chemistry Chemical Physics

CiteScore: 5.5

自己引用率: 10.3%

年間論文数: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Twist–stretch coupling and phase transition during DNA supercoiling

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