Electrochemical behavior of lactate dehydrogenase immobilized on “silica sol–gel/nanometre-sized tridecameric aluminium polycation” modified gold electrode and its application

This paper reports the electrochemical behavior of lactate dehydrogenase (LDH) immobilized in the silica sol–gel film on gold electrode after adding nanometre-sized tridecameric aluminium polycation (nano-Al13, also called nanopolynuclear Al13) as a promoter. A pair of surface controlled quasi-reversible cyclic voltammetry peaks with the formal potential (E0′) of 154 mV (vs.SCE) was found in the presence of nano-Al13. A potential application of the nano-Al13-LDH electrode for the determination of resorcinol and p-xylene was also investigated. The experimental results showed that both resorcinol and p-xylene inhibited LDH activity, and the calibration ranges were 5.0 × 10−6–3.0 × 10−4 mol L−1 for resorcinol and 1.0 × 10−6–1.0 × 10−5 mol L−1 for p-xylene, respectively. The nano-Al13-LDH electrode can be anticipated to be applied to environmental toxic assessments.