Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

文献情報

出版日 2003-07-14

DOI 10.1039/B303949H

インパクトファクター 4.616

著者

Qing Deng, Lisa M. Kauri, Wei-Jun Qian, Gabriella M. Dahlgren, Robert T. Kennedy

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要旨

A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 µm inner diameter by 25 cm long) packed with 7 µm reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content.

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Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

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