Detection and characterization at nM concentration of oligomers formed by hIAPP, Aβ(1–40) and their equimolar mixture using SERS and MD simulations

文献情報

出版日 2018-07-18

DOI 10.1039/C7CP08552D

インパクトファクター 3.676

著者

Luisa D’Urso, Marcello Condorelli, Orazio Puglisi, Carmelo Tempra, Giuseppe Compagnini, Carmelo La Rosa

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要旨

We report a structural investigation on IAPP, Aβ(1–40) and their equi-molar mixture aggregation pathway at nano-molar concentration using the Surface Enhanced Raman Spectroscopy (SERS) effect induced by silver metal colloids prepared by laser processes in solution and molecular dynamics simulations. Our data show the ability of silver NPs coupled with SERS to detect secondary structures of IAPP, Aβ(1–40) and their 1 : 1 molar ratio mixture in the oligomeric state. The preparation of silver colloids shows superior performance with respect to chemically prepared nano-particles. SERS spectroscopy shows both selectivity and sensitivity in detecting the secondary structures of hIAPP and Aβ(1–40) and to recognize both proteins in their mixture. On the other hand, molecular dynamics simulations confirm SERS structural data and the given atomistic details about the structural organization of IAPP and Aβ(1–40) oligomers. Our study shows an inhomogeneity in the chemical composition of IAPP/Aβ(1–40) oligomer aggregates.

掲載誌

Physical Chemistry Chemical Physics

CiteScore: 5.5

自己引用率: 10.3%

年間論文数: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.