A ratiometric fluorescent probe for hydrophobic proteins in aqueous solution based on aggregation-induced emission

文献情報

出版日 2013-01-24

DOI 10.1039/C3AN36634K

インパクトファクター 4.616

著者

Lu Peng, Ruirui Wei, Kai Li, Zhaojuan Zhou, Panshu Song, Aijun Tong

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要旨

A novel fluorescent probe 1 is reported here with ratiometric response to hydrophobic proteins (casein) or proteins with hydrophobic pockets (BSA, HSA) through hydrophobic interaction. Probe 1 underwent deprotonation in aqueous solution at pH 7.4 and emitted blue fluorescence at 436 nm. Upon the addition of BSA, HSA or casein, the aggregation-induced emission fluorescence of 1 at 518 nm was turned on. The fluorescence intensity ratio, I518/I436 was linearly related to the concentrations of these proteins. The detection limits for BSA, HSA and casein based on IUPAC (CDL = 3Sb m−1) were 16.2 μg mL−1, 10.5 μg mL−1 and 5.7 μg mL−1, respectively.

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Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

A ratiometric fluorescent probe for hydrophobic proteins in aqueous solution based on aggregation-induced emission

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