DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices

文献情報

出版日 2012-12-11

DOI 10.1039/C2AN36522G

インパクトファクター 4.616

著者

Peter Friis Østergaard, Marco Matteucci, Walter Reisner, Rafael Taboryski

原文を見る

要旨

Nanochannel technology, coupled with a suitable DNA labeling chemistry, is a powerful approach for performing high-throughput single-molecule mapping of genomes. Yet so far nanochannel technology has remained inaccessible to the broader research community due to high fabrication cost and/or requirement of specialized facilities/skill-sets. In this article we show that nanochannel-based mapping can be performed in all polymer chips fabricated via injection molding: a fabrication process so inexpensive that the devices can be considered disposable. Fluorescent intensity variations can be obtained from molecules extended in the polymer nanochannels via chemical counterstaining against YOYO-1. In particular, we demonstrate that the counterstaining induced fluorescent intensity variations to a large degree appear to be proportional to the theoretically computed sequence-maps of both local AT and GC variation along DNA sequences.

掲載誌

Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices

文献情報

おすすめジャーナル

関連文献

An optimized chromatography method and MC-ICP-MS technique for apatite Lu–Hf geochronology

ICP-MS: a tool for detection and quantitation of fosfomycin residues in cleaning samples of finished product by estimation of phosphorous load

Correction: Local structure and lithium-ion diffusion pathway of cubic Li7La3Zr2O12 studied by total scattering and the Reverse Monte Carlo method

Mechanical, morphological and comparative properties of microbeads assembled from carboxylated cellulose nanocrystals

Synthesis of completely solvent-free biomedical waterborne polyurethane with excellent mechanical property retention and satisfactory water absorption

Protonic nanoenvironment engineering for tuning the electrocatalytic efficiency and product selectivity of O2 reduction

Co-SAC catalyzed utilization of methanol and ethanol in the transfer hydrogenation of azo bonds: experimental and theoretical studies

Rational design of ZIF-derived nanocarbon with dual metal active sites via molten salt strategy for advancing oxygen electrocatalysis

A potential stibnite reference material for sulfur isotope determination by LA-MC-ICP-MS

Zincophilic Sn sites induced the local ion enrichment for compact and homogenous growth of Zn biscuits in long-life Zn metal batteries

こちらもおすすめ

2,3-スチオエポキシマドルを取り扱う際の実験室安全事項は何ですか？

6-氟-2-氨基苯酚の主な用途は何ですか？

BOC-S-3-アミニ-4-(4-メチオキシベンチル)-ブタン酸の代替品はありますか？

Methyl 2-(chloromethyl)-3-nitrobenzoate（1218910-61-2）の代替品はありますか？

（2R)-2-アミノ-N-ベンジル-3-ヒドロキシプロパナミドを含む廃棄物はどのように処理すべきですか？

6,7-二氢-咪唑並[1,2-a]ピリドイン-8(5h)-酮はどのように合成されますか？

エチル(3R)-3-ピロリジニル酢酸水和塩とは何ですか？

（2S）-{[(2-メチルエチルオキシ]カルボニル}アミノ)[2-(トリアフルオロメチルフェニル]エチカシック酸の物理化学的性質は何ですか？

2-ブロモ-1-(2-メチル-2-プロパニル)-4-ニトロベンゼンはどのように保存すればよいですか？

1-[(4-硝基フェニル)スルホニル]-1H-1,2,4-三唑の市場動向や研究トレンドはどうですか？

掲載誌

Analyst

おすすめ化合物

Hexaflumuron

Glycol salicylate

Dimethyl 5-nitroisophthalate

Dipotassium 7-hydroxy-1,3-naphthalenedisulfonate

Danthron (1,8-Dihydroxyanthraquinone)

おすすめサプライヤー