Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

文献情報

出版日 2012-11-23

DOI 10.1039/C2AN36374G

インパクトファクター 4.616

著者

Wei Li, Jie Li, Weibing Qiang, Jingjuan Xu, Danke Xu

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要旨

A novel, enzyme-free and aptamer-based colorimetric platform for protein detection has been developed, which takes advantage of aptamer-functionalized magnetic beads (MBs) for target capture, concentration and separation, and aptamer-conjugated gold nanoparticle (AuNP)-catalyzed color bleaching reaction of methyl orange (MO) to generate the colorimetric signals. It was demonstrated that the proposed colorimetric sensing strategy enables simple, cost-effective, sensitive and specific thrombin detection without the use of any enhancing solutions and enzymes. Herein, by naked eye observation, we can detect the human thrombin with a detection limit of approximately 320 pM, which can be further decreased to 30 pM with the help of a UV-vis instrument. In addition, this method also works for targets with two or more binding sites.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

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