Genetic, genomic and physiological state studies on single-needle bio-electrosprayed human cells

文献情報

出版日 2008-07-02

DOI 10.1039/B806901H

インパクトファクター 4.616

著者

Richard P. Hall, Caroline M. Ogilvie, Emma Aarons, Suwan N. Jayasinghe

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要旨

Bio-electrospraying, a non-contact jet-based direct cell engineering approach, was recently pioneered and demonstrated for handling a wide range of primary living cells. In those studies, post-treated cells were biologically assessed in comparison to several controls by way of flow cytometry. Although flow cytometry accurately assesses those viable populations of cells, subtle effects at a sub-cellular level could have been missed. Therefore, in the present study we demonstrate metaphase chromosome breakage studies carried out on single-needle bio-electrosprayed human T-lymphocytes, which are compared with several controls. The results indicate that post-treated T-lymphocytes do not demonstrate any increase in chromosome damage in comparison to control cells. These studies further validate bio-electrospraying as a technique with potential for clinical utility.

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CiteScore: 7.8

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Genetic, genomic and physiological state studies on single-needle bio-electrosprayed human cells

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