Excited-state dynamics and fluorescence lifetime of cryogenically cooled green fluorescent protein chromophore anions
文献情報
Anne P. Rasmusssen, Henrik B. Pedersen, Lars H. Andersen
Time-resolved action spectroscopy together with a fs-pump probe scheme is used in an electrostatic ion-storage ring to address lifetimes of specific vibrational levels in electronically excited states. Here we specifically consider the excited-state lifetime of cryogenically cooled green fluorescent protein (GFP) chromophore anions which is systematically measured across the S0–S1 spectral region (450–482 nm). A long lifetime of 5.2 ± 0.3 ns is measured at the S0–S1 band origin. When exciting higher vibrational levels in S1, the lifetime changes dramatically. It decreases by more than two orders of magnitude in a narrow energy region ∼250 cm−1 (31 meV) above the 0–0 transition. This is attributed to the opening of internal conversion over an excited-state energy barrier. The applied experimental technique provides a new way to uncover even small energy barriers, which are crucial for excited-state dynamics.
関連文献
A kinetic study of the interaction of DNA with gold nanoparticles: mechanistic aspects of the interaction
Rafael Prado-Gotor, Elia Grueso
DOI: 10.1039/C0CP00901F
Excited state distortions in a charge transfer state of a donor–acceptor [2]rotaxane
Rachel M. Stephenson, Xianghuai Wang, Ali Coskun, J. Fraser Stoddart, Jeffrey I. Zink
DOI: 10.1039/C0CP00801J
Extension of the AMBER force-field for the study of large nitroxides in condensed phases: an ab initio parameterization
Emiliano Stendardo, Alfonso Pedone, Paola Cimino, Maria Cristina Menziani, Orlando Crescenzi, Vincenzo Barone
DOI: 10.1039/C001481H
New insights in the formation of silanol defects in silicalite-1 by water intrusion under high pressure
Mohamed-Ali Saada, Séverinne Rigolet, Anthony Ballandras, Guy Weber, Igor Bezverkhyy, Michel Soulard, Joël Patarin, Jean-Pierre Bellat
DOI: 10.1039/C000931H
Mechanistic studies of the ‘blue’ Cuenzyme, bilirubin oxidase, as a highly efficient electrocatalyst for the oxygenreduction reaction
Victor Climent, Christopher F. Blanford, Fraser A. Armstrong
DOI: 10.1039/C0CP00018C
Electrostatic attraction between a hydrophilic solid and a bubble
Li Jiang, Marta Krasowska, Daniel Fornasiero, Peter Koh, John Ralston
DOI: 10.1039/C0CP01367F
Optical properties of protonated Rhodamine 19 isomers in solution and in the gas phase
Roman M. Balabin, Vladimir Frankevich, Huanwen Chen, Konstantin Barylyuk, Robert Nieckarz, Alexey Fedorov, Renato Zenobi
DOI: 10.1039/C0CP00482K
Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent
Norman R. Watts, Stephen J. Stahl, Paul T. Wingfield, Alasdair C. Steven
DOI: 10.1039/C0CP00692K
The tetragonal structure of nanocrystals in rare-earth doped oxyfluoride glass ceramics
DOI: 10.1039/C0CP00903B
Molecular dynamics simulation for insight into microscopic mechanism of polymer reinforcement
Sizhu Wu, Liqun Zhang, Wenchuan Wang
DOI: 10.1039/C0CP00297F
こちらもおすすめ
6-苄基-6,7-二氢-5H-吡咯并3,4-b吡啶とは何ですか?
6-苄基-6,7-二氢-5H-吡咯并3,4-b吡啶は、CAS番号109966-30-5の化合物です。これは、6-ベンジル基を持つ6,7-二氢-5H-吡咯並みの化...
半硫酸奎宁单水水合物はどのように保存すればよいですか?
半硫酸奎宁单水水合物は、乾燥した涼しい場所に保管し、直射日光や湿気を避ける必要があります。保存温度は常温(15〜25℃)が適切で、湿度は40%以下を維持すること...
D-核糖-5-リン酸二ナトリウムとは何ですか?
D-核糖-5-リン酸二ナトリウムは、CAS番号18265-46-8を有する化合物で、D-核糖の5位付加部位にリン酸基が結合した化合物です。この化合物は、水溶性で...
3-乙酰基-4-羟基喹啉-2(1H)-酮はどのように合成されますか?
3-乙酰基-4-羟基喹啉-2(1H)-酮は、ハイドロキノンと酢酸アセトイルアミドのアミド化反応により合成されます。この反応は塩基触媒を用いて行われ、選択性は良好...
5-溴-4-甲基-1H-吲唑とは何ですか?
5-溴-4-甲基-1H-吲唑は、CAS番号1082041-34-6の化学物質で、化学式はC10H9BrNです。この化合物は淡黄色の結晶性粉末で、吸湿性があります...
3-(4メトキシフェニル)オキテナン-3カーボイル酸の代替品はありますか?
3-(4メトキシフェニル)オキテナン-3カーボイル酸の代替品は、その用途により異なりますが、例えば4-(メトキシフェニル)オキテナン-3カーボイル酸や、他のオキ...
3-イリドオキシピロロ[2,3-b]ピリジン-5-カルボキシlic酸は安全ですか?
3-イリドオキシピロロ[2,3-b]ピリジン-5-カルボキシlic酸は危険な化合物ではありませんが、適切な手袋や保護眼鏡の使用を推奨します。誤って摂取または接触...
3-氟-4- iodobenolを取り扱う際の実験室安全事項は何ですか?
3-氟-4- iodobenolは可燃性を有し、強力な反応性を持つため、取り扱いには注意が必要です。PPE(個人保護具)の着用、ドラフトチャンバーの使用、漏洩時...
掲載誌
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.










![(1R,6R)-6-({[(2-Methyl-2-propanyl)oxy]carbonyl}amino)-3-cyclohexene-1-carboxylic acid structure (1R,6R)-6-({[(2-Methyl-2-propanyl)oxy]carbonyl}amino)-3-cyclohexene-1-carboxylic acid structure](https://static.chemtradehub.com/structs/865/865689-24-3-5fef.webp)

![Methyl 2-[5-(3-Phenoxyphenyl)-2H-tetrazol-2-yl]acetate structure Methyl 2-[5-(3-Phenoxyphenyl)-2H-tetrazol-2-yl]acetate structure](https://static.chemtradehub.com/structs/130/1305320-60-8-84b4.webp)

![trans-2-{[(Tert-butoxy)carbonyl]amino}cyclobutane-1-carboxylic acid structure trans-2-{[(Tert-butoxy)carbonyl]amino}cyclobutane-1-carboxylic acid structure](https://static.chemtradehub.com/structs/951/951173-25-4-27cd.webp)