A homogeneous digital biosensor for circulating tumor DNA by the enumeration of a dual-color quantum dot complex

文献情報

出版日 2021-03-18

DOI 10.1039/D1AN00299F

インパクトファクター 4.616

著者

Xiaojun Liu, Zhangjian Wu, Xinyi Lin, Wei Bu, Lei Qin, Hongwei Gai

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要旨

Monitoring ctDNA in blood is important for cancer management. Here, a one-step single particle counting approach was developed for directly quantifying ctDNA in plasma. Hairpin DNA containing a triple helix stem was immobilized onto QD 585 as a probe. The hairpin was opened by the target, and therefore hybridized with assistant DNA on QD 655, resulting in an aggregate of QD 585 and QD 655. The two-color QD aggregate was regarded as the target. Observed under a single particle transmission grating-based spectral microscope, the two-color QD aggregate was distinguished by a unique spectral pattern of two first-order streaks, and it was counted. The difference in the responses of the probes to perfect-match DNA, single-base mismatch DNA, and non-match DNA indicated that the probe had sufficient single-base discrimination capabilities. The success in plasma recovery tests demonstrated the feasibility of carrying out the direct detection of ctDNA in plasma.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

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A homogeneous digital biosensor for circulating tumor DNA by the enumeration of a dual-color quantum dot complex

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