Transient IR spectroscopy identifies key interactions and unravels new intermediates in the photocycle of a bacterial phytochrome
文献情報
Joachim Kübel, Manoop Chenchiliyan, Saik Ann Ooi, Emil Gustavsson, Linnéa Isaksson, Valentyna Kuznetsova, Janne A. Ihalainen, Sebastian Westenhoff, Michał Maj
Phytochromes are photosensory proteins in plants, fungi, and bacteria, which detect red- and far-red light. They undergo a transition between the resting (Pr) and photoactivated (Pfr) states. In bacterial phytochromes, the Pr-to-Pfr transition is facilitated by two intermediate states, called Lumi-R and Meta-R. The molecular structures of the protein in these states are not known and the molecular mechanism of photoconversion is not understood. Here, we apply transient infrared absorption spectroscopy to study the photocycle of the wild-type and Y263F mutant of the phytochrome from Deinococcus radiodurans (DrBphP) from nano- to milliseconds. We identify two sequentially forming Lumi-R states which differ in the local structure surrounding the carbonyl group of the biliverdin D-ring. We also find that the tyrosine at position 263 alters local structure and dynamics around the D-ring and causes an increased rate of Pfr formation. The results shed new light on the mechanism of light-signalling in phytochrome proteins.
関連文献
An efficient particulate photocatalyst for overall water splitting based on scandium and magnesium co-doped strontium titanate
Riku Okamoto, Akira Kimura, Yuhi Nakayasu, Akira Yamakata, Ryota Tomizawa, Taizo Masuda, Koichiro Nakatani
DOI: 10.1039/D3SE01408H
Unravelling the band splitting origin in bulk and 2D distorted α-CsPbI3 perovskite
Safieh Nazari, Fatemeh Mohammad Dezashibi, Nadia Babaei Bidmeshki
DOI: 10.1039/D3CP04558G
The effect of weak π–π interactions on single-molecule electron transport properties of the tetraphenylethene molecule and its derivatives: a first-principles study
Zhiye Wang, Yunchuan Li, Mingjun Sun
DOI: 10.1039/D3CP04593E
Breaking the size constraint for nano cages using annular patchy particles
Vikki Anand Varma, Simmie Jaglan, Mohd Yasir Khan, Sujin B. Babu
DOI: 10.1039/D3CP03681B
Aging of quinoxaline-based polymer solar cells under UV-free white light
Martin Hager, Frédéric Laquai, Yingping Zou
DOI: 10.1039/D3SE00987D
First principles study of a triazine-based covalent organic framework as a high-capacity anode material for Na/K-ion batteries
Sitong Liu, Bo Liu, Meidong Yu, Hanyu Gao, Haipeng Guo, Daguo Jiang, Shenbo Yang, Yufeng Wen, Yabei Wu
DOI: 10.1039/D3CP04721K
From brew to clean fuel: harnessing distillery wastewater for electrolysis H2 generation using nano scale nickle selenide water oxidation catalysts
Michael Walsh, Jeannie Z. Y. Tan, Sanjay Nagarajan, Kenneth Macgregor, John M. Andresen, M. Mercedes Maroto-Valer, Sudhagar Pitchaimuthu
DOI: 10.1039/D3SE01445B
Novel porous Al-based composites for improved Al–water reaction performances by spark plasma sintering
Jinfan Wu, Tao Wang, Fen Xu, Lixian Sun, Yuan Gao, Hehui Wang, Guorong Zhang, Zhong Cao, Julan Zeng
DOI: 10.1039/D3SE01162C
The single metal atom (Ni, Pd, Pt) anchored on defective hexagonal boron nitride for oxidative desulfurization
Naixia Lv, Hongshun Ran, Jinrui Zhang, Jie Yin, Yuan Zhang, Hongping Li, Linhua Zhu
DOI: 10.1039/D3CP04963A
Reinforcement learning-based control for the thermal management of the battery and occupant compartments of electric vehicles
Jianglu Huang, Donggang Zhao, Yu Zhao
DOI: 10.1039/D3SE01403G
こちらもおすすめ
2-メトキシ-4-(メチルスルフィニル)アミンの主な用途は何ですか?
2-メトキシ-4-(メチルスルフィニル)アミンは、主に医薬品および農薬の製造に使用されます。また、合成化学の一部として研究用材料としても利用されます。
4,6-二氯-N-甲基ピラミジンアミンの代替品はありますか?
代替品としては、4,6-二クロロピラミジンアミンや他のピラミジン系化合物が考えられます。ただし、目的と用途によって最適な代替品は異なります。
6-氯-4-甲基-1H-吲哚を含む廃棄物はどのように処理すべきですか?
6-氯-4-甲基-1H-吲哚の廃棄物は、適切な容器に収集し、密閉して保管します。温度は常温、湿度は低く、直射日光を避けて保管することを推奨します。廃棄処理は専門...
2-フローユロ-4-(トリフルオロメチル)ベンゾイドについて「に適用される法規ガイドラインは何ですか」
2-フローユロ-4-(トリフルオロメチル)ベンゾイドのCAS番号は207974-08-1です。この化合物はGHS分類で毒性物質と有害な反応物質として分類されます...
4-ニトロフェニルN-[(ベンゼルオキシルカーボンイル]グリシングリシングリシン酸はどのように保存すればよいですか?
4-ニトロフェニルN-[(ベンゼルオキシルカーボンイル]グリシングリシングリシン酸は、室温で暗所に保管し、乾燥した環境で保存することを推奨します。容器は密閉性の...
イソデスロラタドリンの代替品はありますか?
イソデスロラタドリンの代替品としては、デスロラタドリンや他の抗ヒスタミン薬が挙げられます。具体的には、デスロラタドリン、ラセカミド、フェルタドリンなどが、症状や...
5-甲氧基-1,2,3,4-四氢异喹啉盐酸盐はどのように合成されますか?
5-甲氧基-1,2,3,4-四氢异喹啉盐酸盐の一般的な合成方法は、メタノール中で5-メトキシ-1,2,3,4-四ヒュドロイソキシンを塩酸で塩化します。この反応で...
4-アミノ-5-メトキシ-2-トルエンサルホニック酸についての法規ガイドラインは何ですか?
CAS番号6471-78-9の4-アミノ-5-メトキシ-2-トルエンサルホニック酸は、GHS分類では corrosive(腐食性)と識別されます。EUのREAC...
甲基孕酮を取り扱う際の実験室安全事項は何ですか?
甲基孕酮の取り扱いは、PPE(個人保護具)の使用が必要な重要な安全事項を伴います。防塵マスク、ゴーグル、手袋を着用することが推奨されます。ドラフトチャンバーを使...
掲載誌
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.










![2-Methylbenzo[h]quinoline structure 2-Methylbenzo[h]quinoline structure](https://static.chemtradehub.com/structs/605/605-88-9-ac43.webp)



