A fast and validated capillary zone electrophoresis method for the determination of selected fatty acids applied to food and cosmetic purposes

文献情報

出版日 2019-10-08

DOI 10.1039/C9AY01917K

インパクトファクター 2.896

著者

Tatiane Lima Amorim, Mariana Gavioli dos Reis Pena, Fabiano Freire Costa, Marcone Augusto Leal de Oliveira, Paula Rocha Chellini

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要旨

Fatty acids (FAs) are classically determined by gas chromatography (GC), but capillary electrophoresis (CE) can be used to determine the FA fingerprint in only a few minutes by employing small amounts of reagents and solvents without requiring specific columns for analysis. In this study, palmitic (C16:0), stearic (C18:0), oleic (C18:1 c9) and linoleic (C18:2 c9c12) FAs were determined in pumpkin seed oil and a moisturizing cream containing this oil by capillary zone electrophoresis under indirect ultraviolet absorption detection (CZE-UV), showing the potential of CE for FA determination in challenging matrices. The method was validated with regards to limits of detection and quantification (LOD and LOQ), linearity, accuracy and repeatability. The method may be used for the fast monitoring of FAs in food and cosmetics, and also for labelling purposes. The LOD for C16:0, C18:0, C18:1 c9 and C18:2 c9c12 were 0.03, 0.05, 0.04 and 0.04 mmol L−1, respectively, and the LOQ was 0.1 mmolL−1 for all FAs. No significant difference was found between the classical GC and this alternative method within the 95% confidence interval. Furthermore, the repeatability tests showed minor relative standard deviations for the migration time, evidencing the precision of this CZE-UV method.

掲載誌

Analytical Methods

CiteScore: 5.1

自己引用率: 3.7%

年間論文数: 655

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development