Preparation and application of molecularly imprinted polymers for the selective extraction of naringin and genistein from herbal medicines

文献情報

出版日 2019-08-28

DOI 10.1039/C9AY01503E

インパクトファクター 2.896

著者

Zhimin Luo, Aiping Xiao, Guoning Chen, Qi Guo, Chun Chang, Aiguo Zeng, Qiang Fu

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要旨

Flavonoids are a class of important bioactive chemical constituents in herbal medicines and the simple, fast and selective extraction of flavonoids from herbal samples is quite meaningful. In this study, a simple and selective sample pretreatment procedure coupling molecularly imprinted polymers and solid-phase extraction (MIPs-SPE) was developed for the extraction and purification of flavonoids from herbal medicine samples. MIPs for flavonoids were synthesized through a multi-step swelling and polymerization method, using 4-vinylpyridine as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. Scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis were used to characterize the structural features of the obtained MIPs. The recognition performance of the polymers was investigated and several parameters affecting the recognition performance were also studied including the pH value of the mobile phase, the ratio of acetonitrile, the column temperature, the injection volume and the flow-rate of the mobile phase. The results revealed that a relatively good mono-dispersity of MIPs was obtained, and the average size of the obtained polymers was about 3–5 μm. Under the optimum recognition conditions, the obtained MIPs were successfully employed as selective adsorbents for SPE for the specific recognition and purification of flavonoids from herbal medicine samples.

掲載誌

Analytical Methods

CiteScore: 5.1

自己引用率: 3.7%

年間論文数: 655

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development