Development of an endoplasmic reticulum-targeting fluorescent probe for the two-photon imaging of hypochlorous acid (HClO) in living cells

文献情報

出版日 2019-08-03

DOI 10.1039/C9AY01390C

インパクトファクター 2.896

著者

Wenhui Song, Baoli Dong, Yaru Lu, Xiuqi Kong, Abdul Hadi Mehmood, Weiying Lin

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要旨

As an important reactive oxygen species, hypochlorous acid (HClO) could irreversibly destroy Ca2+-ATPase to inhibit Ca2+ transport in the endoplasmic reticulum (ER), which may eventually lead to cell dysfunction and cell death. Herein, we present a novel ER-targeting fluorescent probe (NHS-ER) for the two-photon detection of HClO in living cells. NHS-ER was constructed using naphthalimide and showed strong green fluorescence. In response to HClO, the fluorescence intensity at 502 nm gradually decreased, and the probe showed excellent selectivity to HClO over other biologically relevant ROS. Moreover, the probe NHS-ER showed ER-targeting properties and could be successfully applied to the two-photon imaging of HClO in living cells.

掲載誌

Analytical Methods

CiteScore: 5.1

自己引用率: 3.7%

年間論文数: 655

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development

Development of an endoplasmic reticulum-targeting fluorescent probe for the two-photon imaging of hypochlorous acid (HClO) in living cells

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