An efficient method for high-pH peptide fractionation based on C18 StageTips for in-depth proteome profiling

文献情報

出版日 2019-08-20

DOI 10.1039/C9AY01269A

インパクトファクター 2.896

著者

Kisoon Dan, Hyunsuk Shin, Junghun Lee, Joseph Injae Wang, Dohyun Han

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要旨

In-depth proteome profiling still relies widely on extensive off-line peptide fractionation to reduce the complexity of the proteome. However, the fractionation of protein samples in the sub-microgram range can be limited owing to sample loss during the fractionation process. Thus, it is essential to develop efficient microscale fractionation strategies. Here, we describe a modified method for high-pH peptide fractionation using C18-StageTips. Compared to a conventional strategy, our method generates improved peptide and protein identification rates under the same LC-MS/MS conditions. Finally, proteome profiling of sub-regions of the mouse brain suggested that our method can be used to analyze the proteome in-depth with limited amounts of protein samples.

掲載誌

Analytical Methods

CiteScore: 5.1

自己引用率: 3.7%

年間論文数: 655

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development

An efficient method for high-pH peptide fractionation based on C18 StageTips for in-depth proteome profiling

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