Specific protein-labeling and ligand-binding position analysis with amidopyrene probes as LDI MS tags

文献情報

出版日 2018-10-04

DOI 10.1039/C8OB02222D

インパクトファクター 3.876

著者

Rei Watanabe, Yaping Hu, Keita Iio, Kozo Yoneda, Atsunori Hattori, Atsushi Arai, Hideo Kigoshi

原文を見る

要旨

To readily analyze the binding mode of protein–ligand interactions, we developed ligand-bound-type and ligand-dissociation-type probes having 6-amidopyrene (apy) as a detecting group. Matrix- and label-assisted laser desorption/ionization mass spectrometry (MALDI and LA-LDI MS) analyses and a covalent docking simulation using these probes precisely determined the binding position of the ligand biotin on the target protein avidin (RMSD = 0.786 and 0.332 Å). Our apy-probe-labeling method may be useful for determining the unknown ligand-binding sites of various target proteins.

掲載誌

Organic & Biomolecular Chemistry

CiteScore: 3.4

自己引用率: 10.3%

年間論文数: 1041

Organic & Biomolecular Chemistry (OBC) publishes original and high impact research and reviews in organic chemistry. We welcome research that shows new or significantly improved protocols or methodologies in total synthesis, synthetic methodology or physical and theoretical organic chemistry as well as research that shows a significant advance in the organic chemistry or molecular design aspects of chemical biology, catalysis, supramolecular and macromolecular chemistry, theoretical chemistry, mechanism-oriented physical organic chemistry, medicinal chemistry or natural products. Articles published in the journal should report new work which makes a highly-significant impact in the field. Routine and incremental work is generally not suitable for publication in the journal. More details about key areas of our scope are below. In all cases authors should include in their article clear rationale for why their research has been carried out.

Specific protein-labeling and ligand-binding position analysis with amidopyrene probes as LDI MS tags

文献情報

おすすめジャーナル

関連文献

Energy-directed tree search: an efficient systematic algorithm for finding the lowest energy conformation of molecules

Compositional analysis of copper–silica precipitation tubes

On the σ,π-energy separation of the aromatic stabilization energy of cyclobutadiene

Inside front cover

Back matter

Inelastic insights for molecular tunneling pathways: Bypassing the terminal groups

Temperature effects on femtosecond transient absorption kinetics of semiconducting single-walled carbon nanotubes

Controlling crystallization and its absence: proteins, colloids and patchy models

Back cover

Short-range interactions between non-ionic surfactant layers

こちらもおすすめ

3-イチチルビフェニルはどのように合成されますか？

8-溴-5-三氟甲基喹啉はどのように合成されますか？

ジメチル4-(4,4,5,5-テトラメチル-1,3,2-ドioxaborolan-2-基)-2,6-ピリジンジカルボイル酸フェニルアミニドの代替品はありますか？

N-(3,5-ヘキサクロロ-4-ピリドインイル)-8-メチオキシ-5-キノリンカーボン酸の市場動向や研究トレンドはどのようなものでしょうか？

イソステアロイルグリセリルは安全ですか？

1-(二苯甲基)-3,3-二氟-氮杂环丁烷の市場動向や研究トレンドはどうですか？

3-チオフェンスチオールの物理化学的性質は何ですか？

2-Methyl-2-propanyl (2S)-2-(aminomethyl)-1-piperidinecarboxylateは安全ですか？

CAS番号1316822-90-8の化合物は安全ですか？

Tert-butyl 2-(2-羟基乙基)哌嗪-1-羧酸はどのように保存すればよいですか？

掲載誌

Organic & Biomolecular Chemistry

おすすめ化合物

Isomorellinol

5'-Fluoro-[2,3'-biindolinylidene]-2',3-dione

8-Bromo-7-fluoro-2-methoxyquinoline

1,10-bis(3,5-dimethylphenyl)-12-hydroxy-4,5,6,7-tetrahydroiindeno[7,1-de:1',7'-fg][1,3,2]dioxaphosphocine 12-oxide

(2R)-2-(3-Bromophenyl)pyrrolidine hydrochloride (1:1)

おすすめサプライヤー