Time-resolved IR spectroscopy reveals mechanistic details of ion transport in the sodium pump Krokinobacter eikastus rhodopsin 2
文献情報
Marvin Asido, Peter Eberhardt, Clara Nassrin Kriebel, Markus Braun, Clemens Glaubitz, Josef Wachtveitl
We report a comparative study on the structural dynamics of the light-driven sodium pump Krokinobacter eikastus rhodopsin 2 wild type under sodium and proton pumping conditions by means of time-resolved IR spectroscopy. The kinetics of KR2 under sodium pumping conditions exhibits a sequential character, whereas the kinetics of KR2 under proton pumping conditions involves several equilibrium states. The sodium translocation itself is characterized by major conformational changes of the protein backbone, such as distortions of the α-helices and probably of the ECL1 domain, indicated by distinct marker bands in the amide I region. Carbonyl stretch modes of specific amino acid residues helped to elucidate structural changes in the retinal Schiff base moiety, including the protonation and deprotonation of D116, which is crucial for a deeper understanding of the mechanistic features in the photocycle of KR2.
おすすめジャーナル

Lab on a Chip

Physical Chemistry Chemical Physics

Journal of Medical Biochemistry

Contact Lens & Anterior Eye

Angewandte Chemie International Edition

Mini-Reviews in Medicinal Chemistry

Advanced Engineering Materials

Environmental Toxicology and Pharmacology

Faraday Discussions

Journal of Enzyme inhibition and Medicinal Chemistry
関連文献
Translocation of copper within the cavity of cryptands: reversible fluorescence signaling
Kalyan K. Sadhu, Parimal K. Bharadwaj
DOI: 10.1039/B806426A
Sequence-specifically platinum metal deposition on enzymatically synthesized DNA block copolymer
Aya Tanaka, Yasutaka Matsuo, Yuichi Hashimoto, Kuniharu Ijiro
DOI: 10.1039/B805989F
Binary stacks of [CuC6F5]4 with arenes
Ami Doshi, Krishnan Venkatasubbaiah, Arnold L. Rheingold, Frieder Jäkle
DOI: 10.1039/B807128D
Microwave effect on the surface composition of the Urushibara Ni hydrogenation catalyst and improved reduction of acetophenone
Junichi Tsuzuki, Futoshi Sakai, Masatsugu Kajitani, Nick Serpone
DOI: 10.1039/B808188C
Transistors from a conjugated macrocycle molecule: field and photo effects
Wei Zhao, Qin Tang, Hoi Shan Chan, Jianbin Xu, Ka Yuen Lo, Qian Miao
DOI: 10.1039/B806601A
Lewis base-catalyzed conjugate reduction and reductive aldol reaction of α,β-unsaturated ketones using trichlorosilane
Masaharu Sugiura, Norimasa Sato, Shunsuke Kotani, Makoto Nakajima
DOI: 10.1039/B807529H
Fabrication of mesoporous Pt nanotubes utilizing dual templates under a reduced pressure condition
Azusa Takai, Yusuke Yamauchi, Kazuyuki Kuroda
DOI: 10.1039/B804072A
Silica-supported catalysts for ring-closing metathesis: effects of linker group and microenvironment on recyclability
Jaehong Lim, Su Seong Lee, Jackie Y. Ying
DOI: 10.1039/B803663B
Controlled generation of acentric and homochiral coordination compounds from a versatile asymmetric ligand 4-(1H-1,2,4-triazol-3-yl)-4H-1,2,4-triazole
Jian Zhang, Zhao-Ji Li, Ye-Yan Qin, Jian-Kai Cheng, Yuan-Gen Yao
DOI: 10.1039/B807320A
Diol-substituted boron complexes of dipyrrolyl diketones as anion receptors and covalently linked ‘pivotal’ dimers
Yasunobu Fujii, Yuta Mihashi
DOI: 10.1039/B806361C
こちらもおすすめ
2-ブロモ-9,9-ジフェニル-9H-フルオレンの主な用途は何ですか?
2-溴-9,9-二苯基芴は、医薬品、工業材料、有機合成の研究分野で応用されます。特に、レーザー材料や機能性ポリマーの合成に使用されることがあります。また、蛍光色...
四氯化铱の市場動向や研究トレンドはどうですか?
四氯化铱の市場は研究開発分野で注目されており、特にナノ技術や金属有機框架(MOFs)の分野での需要が増加傾向にあります。価格は安定しており、中国や韓国での生産が...
1-(4-溴-3-氟苯基)-2-氯乙酮を含む廃棄物はどのように処理すべきですか?
1-(4-溴-3-氟苯基)-2-氯乙酮 (CAS番号: 1260857-14-4) の廃棄物は専門的な廃棄処理が必要です。まず、廃棄物は密閉された容器に収集し、...
苦参酚Kとは何ですか?
苦参酚Kは、CAS番号101236-49-1を持つ化合物で、主に天然由来の生薬から抽出されます。この化合物は、抗炎症作用や抗癌作用を持つことが報告されています。
POTASSIUM (1-(TERTBUTOXYCARBONYL)AZETIDIN-3-YL)TRIFLUOROBORATE を含む廃棄物はどのように処理すべきですか?
POTASSIUM (1-(TERTBUTOXYCARBONYL)AZETIDIN-3-YL)TRIFLUOROBORATE を含む廃棄物は、まず安全なエント...
4-庚基-4’-联苯羧酸の市場動向や研究トレンドはどうですか?
4-庚基-4’-聯苯羧酸は、特殊化学品や合成化学の分野で用いられる化学物質ですが、市場動向としては、研究開発の進展とともに需要が増加しています。また、環境配慮型...
6-ブロモ-3-メトキシ-1-フェニル-1H-インドゾールを含む廃棄物はどのように処理すべきですか?
6-ブロモ-3-メトキシ-1-フェニル-1H-インドゾールを含む廃棄物は、適切な化学廃棄処理が必要です。通常、廃棄物は密閉容器に収集され、専門の廃棄処理業者に引...
4,4-二甲基-2-吡咯烷酮はどの業界で使用されていますか?
4,4-二甲基-2-吡咯烷酮は医薬、ポリマー、センサー、半導体などの業界で広く使用されています。特に溶媒としての性能が高く評価されています。
掲載誌
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.
![4-Chloro-3-(trifluoromethyl)-1H-pyrrolo[2,3-b]pyridine structure 4-Chloro-3-(trifluoromethyl)-1H-pyrrolo[2,3-b]pyridine structure](https://static.chemtradehub.com/structs/869/869335-75-1-a9d0.webp)

![2,6-Di(thiophen-2-yl)dithieno[3,2-b:2',3'-d]thiophene structure 2,6-Di(thiophen-2-yl)dithieno[3,2-b:2',3'-d]thiophene structure](https://static.chemtradehub.com/structs/910/910788-24-8-5b70.webp)
![6-Benzyl-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridin-3(2H)-one structure 6-Benzyl-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridin-3(2H)-one structure](https://static.chemtradehub.com/structs/909/909187-64-0-f54f.webp)
