Comparison of catalysis by haloalkane dehalogenases in aqueous solutions of deep eutectic and organic solvents
文献情報
Radka Chaloupkova
Haloalkane dehalogenases catalyze the hydrolytic cleavage of carbon–halogen bonds in diverse halogenated hydrocarbons and are attractive catalysts for sustainable biotechnologies. However, their use in industrial processes is limited due to the poor water solubility of their substrates and the tendency of the substrates to undergo abiotic hydrolysis. Here we systematically and critically compare the performance of three haloalkane dehalogenases, DbjA, DhaA and LinB, in aqueous solutions of the deep eutectic solvent ethaline, its components (ethylene glycol and choline chloride), and two organic solvents (methanol and acetone). Each of the solvents had different effects on the activity of each enzyme. Haloalkane dehalogenase DhaA was found to be the most tolerant to ethaline, retaining 21% of its reference activity even in solutions containing 90% (v/v) of ethaline. However, dissolution in 75% (v/v) ethylene glycol, 50% (v/v) methanol, or 25% (v/v) acetone caused almost total loss of DhaA activity. In contrast, the activities of DbjA and LinB were higher in ethylene glycol than in ethaline, and moreover the activity of DbjA was 1.5 times higher in 50% (v/v) ethylene glycol than in pure buffer. Interestingly, the enantioselectivity of 2-bromopentane hydrolysis catalysed by DbjA increased more than 4-fold in the presence of ethaline or ethylene glycol. Our results demonstrate that ethylene glycol and an ethylene glycol-based deep eutectic solvent can have beneficial effects on catalysis by haloalkane dehalogenases, broadening their usability in “green” biotechnologies.
関連文献
Mechano-switchable, luminescent gels derived from salts of a long-chained, fatty-acid gelator
Mohan Zhang
DOI: 10.1039/C6CP03435G
Unravelling the mechanisms of reactive oxygen species formation in nanohybrid systems of porphyrins and enriched (6,5) single-walled carbon nanotubes for photosensitization
Camila S. Monteiro, Daniele C. Ferreira, Gustavo A. M. Sáfar, Rafael N. Gontijo, Cristiano Fantini, Dayse C. S. Martins, Ynara M. Idemori, Maurício V. B. Pinheiro, Klaus Krambrock
DOI: 10.1039/C6CP03366K
Role of donor–acceptor macrocycles in sequence specific peptide recognition and their optoelectronic properties: a detailed computational insight
Arkamita Bandyopadhyay
DOI: 10.1039/C6CP03181A
Flexible band gap tuning of hexagonal boron nitride sheets interconnected by acetylenic bonds
Hongyu Zhang, Youhua Luo, Xiaojuan Feng, Lixia Zhao, Meng Zhang
DOI: 10.1039/C5CP02346G
Computational insights into CdSe quantum dots' interactions with acetate ligands
Patrick K. Tamukong, Wadumesthrige D. N. Peiris, Svetlana Kilina
DOI: 10.1039/C6CP01665K
Photoionization access to cyclodextrin-encapsulated resveratrol phenoxy radicals and their repair by ascorbate across the phase boundary
Christoph Kerzig, Martin Goez
DOI: 10.1039/C6CP03742A
Nanoscale magnetism and novel electronic properties of a bilayer bismuth(111) film with vacancies and chemical doping
Yajun Zhang
DOI: 10.1039/C6CP03056D
Double deuterated acetylacetone in neon matrices: infrared spectroscopy, photoreactivity and the tunneling process
Michèle Chevalier, Claudine Crépin
DOI: 10.1039/C6CP02796B
Internal conversion and intersystem crossing pathways in UV excited, isolated uracils and their implications in prebiotic chemistry
Hui Yu, Jose A. Sanchez-Rodriguez, Marvin Pollum, Carlos E. Crespo-Hernández, Sebastian Mai, Philipp Marquetand, Leticia González, Susanne Ullrich
DOI: 10.1039/C6CP01790H
New organophilic kaolin clays based on single-point grafted 3-aminopropyl dimethylethoxysilane
A. Zaharia, F.-X. Perrin, M. Teodorescu, A.-L. Radu, T.-V. Iordache, A.-M. Florea, D. Donescu, A. Sarbu
DOI: 10.1039/C5CP04395F
こちらもおすすめ
4-アミノフェノール酸ナトリウム水和物とは何ですか?
4-アミノフェノール酸ナトリウム水和物は、CAS番号206557-08-6の化合物で、4-アミノフェノールとナトリウムが結合した塩と水和物です。この化合物は、白...
Methyl 3-methyl-N-{[(2-methyl-2-propanyl)oxy]carbonyl}-L-histidinateの代替品はありますか?
この化合物は特定の合成プロセスに使用される可能性がありますが、代替品として、他の类似的な化合物、例えばMethyl 3-methyl-N-{[(2-methyl...
4-Boc-2-哌嗪甲酸の市場動向や研究トレンドはどうですか?
4-Boc-2-哌嗪甲酸は、薬品開発や合成化学分野で広く使用されており、その需要は継続的に推移しています。特に、新薬開発における合成化学分野での需要が高まってい...
4,4'-二羟甲基联苯の物理化学的性質は何ですか?
4,4'-二羟甲基联苯のCAS番号は1667-12-5です。この化合物は白色の結晶粉末で、分子量は154.20です。水にわずかに溶けますが、アルコールや有機溶媒...
5-甲硫基戊腈はどの業界で使用されていますか?
5-甲硫基戊腈は医薬品産業で使用される可能性があります。また、ポリマー合成の触媒として、センサー製造の一部として、半導体製造のプロセス改善に使用される可能性があ...
CAS番号1311961-50-8の化合物はどのように合成されますか?
この化合物は、1-abieta-8,11,13-trien-19-イルと6'-メトキシシンコナナン-9-基を含有する窒素含有化合物から合成されます。一般的な合成...
6-ブロモベンジジミダゾール-2-カルビルデオキシドはどのように保存すればよいですか?
6-ブロモベンジジミダゾール-2-カルビルデオキシドは、避光・乾燥した容器(密閉容器)で-20℃~4℃の低温で保存してください。高温や直射日光、湿気は避けてくだ...
Boc-N-甲基氨甲环酸とは何ですか?
621-65-8のCAS番号を持つBoc-N-甲基氨甲环酸は、化学式C7H13NO5を有する化合物です。この化合物は白色の結晶性粉末で、吸湿性があります。
乙基三氟硼酸钾はどのように合成されますか?
乙基三氟硼酸钾は、トリフLUオール酸カリウムとエチルブロミドを反応させて合成されます。この反応は高い選択性と収率を持ち、触媒を用いることで効率的に進行します。
2-フロウロ-5-クロロ-4-アミノフェノールはどのように保存すればよいですか?
2-フロウロ-5-クロロ-4-アミノフェノールは、直射日光を避けて冷却された暗所で保存し、密閉容器に保管してください。温度は常温か低温が適しています。
掲載誌
Green Chemistry

Green Chemistry provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on, but not limited to, the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998). Green chemistry is the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry is at the frontiers of this continuously-evolving interdisciplinary science and publishes research that attempts to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. Submissions on all aspects of research relating to the endeavour are welcome. The journal publishes original and significant cutting-edge research that is likely to be of wide general appeal. To be published, work must present a significant advance in green chemistry. Papers must contain a comparison with existing methods and demonstrate advantages over those methods before publication can be considered. For more information please see this Editorial. Coverage includes the following, but is not limited to: Design (e.g. biomimicry, design for degradation/recycling/reduced toxicity…) Reagents & Feedstocks (e.g. renewables, CO2, solvents, auxiliary agents, waste utilization…) Synthesis (e.g. organic, inorganic, synthetic biology…) Catalysis (e.g. homogeneous, heterogeneous, enzyme, whole cell…) Process (e.g. process design, intensification, separations, recycling, efficiency…) Energy (e.g. renewable energy, fuels, photovoltaics, fuel cells, energy storage, energy carriers…) Applications (e.g. electronics, dyes, consumer products, coatings, pharmaceuticals, preservatives, building materials, chemicals for industry/agriculture/mining…) Impact (e.g. safety, metrics, LCA, sustainability, (eco)toxicology…) Green chemistry is, by definition, a continuously-evolving frontier. Therefore, the inclusion of a particular material or technology does not, of itself, guarantee that a paper is suitable for the journal. To be suitable, the novel advance should have the potential for reduced environmental impact relative to the state of the art. Green Chemistry does not normally deal with research associated with 'end-of-pipe' or remediation issues.










![6,7-Dihydro-5H-pyrrolo[1,2-a]imidazole-6-carboxylic acid structure 6,7-Dihydro-5H-pyrrolo[1,2-a]imidazole-6-carboxylic acid structure](https://static.chemtradehub.com/structs/136/1369160-12-2-6524.webp)
![(4aR,5S,6R,8aS)-5-[2-(3-Furyl)ethyl]-8a-(hydroxymethyl)-5,6-dimethyl-3,4,4a,5,6,7,8,8a-octahydro-1-naphthalenecarboxylic acid structure (4aR,5S,6R,8aS)-5-[2-(3-Furyl)ethyl]-8a-(hydroxymethyl)-5,6-dimethyl-3,4,4a,5,6,7,8,8a-octahydro-1-naphthalenecarboxylic acid structure](https://static.chemtradehub.com/structs/184/18411-75-1-d4cd.webp)


![[3-(2,6-Dichlorophenyl)-5-isopropyl-1,2-oxazol-4-yl]methanol structure [3-(2,6-Dichlorophenyl)-5-isopropyl-1,2-oxazol-4-yl]methanol structure](https://static.chemtradehub.com/structs/278/278597-30-1-5c79.webp)