Preparation of molecularly imprinted polymers for the recognition of proteins via the generation of peptide-fragment binding sites by semi-covalent imprinting and enzymatic digestion

文献情報

出版日 2015-01-28

DOI 10.1039/C4AN02299H

インパクトファクター 4.616

著者

Hironori Taguchi, Hirobumi Sunayama, Eri Takano, Yukiya Kitayama, Toshifumi Takeuchi

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要旨

Molecularly imprinted polymers bearing peptide fragment-based binding sites within the protein-imprinted cavities were prepared by copolymerization of the acrylated protein with 6-monoacryloyl-trehalose and 6,6′-diacryloyl-trehalose as a hydrophilic comonomer and a crosslinker respectively, followed by enzymatic decomposition of the grafted protein into the polymer matrix with pepsin, resulting in the creation of peptide fragment-based protein-binding sites.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

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Preparation of molecularly imprinted polymers for the recognition of proteins via the generation of peptide-fragment binding sites by semi-covalent imprinting and enzymatic digestion

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