A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

文献情報

出版日 2014-12-10

DOI 10.1039/C4AN02064B

インパクトファクター 4.616

著者

Hisakage Funabashi, Keisuke Nakatsuka, Akio Kuroda

原文を見る

要旨

A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure from open to closed states and produces a FRET signal in response to in vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 mRNA as a pre-fixed target mRNA model and Hes-1 mRNA as a model expressed inside a living cell. These results confirm the feasibility of using the FRET-based DNA-NT for imaging analysis of target mRNA.

掲載誌

Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

文献情報

おすすめジャーナル

関連文献

A new simple synthesis of poly(thiophene-methine)s

Photochemical release of nitric oxide from a regenerable, sol-gel encapsulated Ru–salen–nitrosyl complex

Shape-dependent magnetic properties of low-dimensional nanoscale Prussian blue (PB) analogue SmFe(CN)6·4H2O

Comb-shaped poly(ethylene glycol)-modified subtilisin Carlsberg is soluble and highly active in ionic liquids

A latent photoreaction predominates within water-soluble calixarenes: photochemistry of benzoinalkyl ethers

The first ternary tin(ii) nitride: NaSnN

Amphiphilic p-sulfonatocalix[4]arene-coated CdSe/ZnS quantum dots for the optical detection of the neurotransmitter acetylcholine

Lower rim mono-functionalization of resorcinarenes

Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-l-LNA

Gold supported on a mesoporous CeO2matrix as an efficient catalyst in the selective aerobic oxidation of aldehydes in the liquid phase

こちらもおすすめ

2-メトキシ-4-(メチルスルフィニル)アミンの主な用途は何ですか？

4-溴甲基-3-甲氧基苯甲酸は安全ですか？

4,6-二氯-N-甲基ピラミジンアミンの代替品はありますか？

6-氯-4-甲基-1H-吲哚を含む廃棄物はどのように処理すべきですか？

2-フローユロ-4-(トリフルオロメチル)ベンゾイドについて「に適用される法規ガイドラインは何ですか」

4-ニトロフェニルN-[(ベンゼルオキシルカーボンイル]グリシングリシングリシン酸はどのように保存すればよいですか？

イソデスロラタドリンの代替品はありますか？

5-甲氧基-1,2,3,4-四氢异喹啉盐酸盐はどのように合成されますか？

4-アミノ-5-メトキシ-2-トルエンサルホニック酸についての法規ガイドラインは何ですか？

甲基孕酮を取り扱う際の実験室安全事項は何ですか？

掲載誌

Analyst

おすすめ化合物

L-(4-~13~C)Asparagine hydrate (1:1)

4-Chloro-2-{[(2-chlorophenoxy)acetyl]amino}benzoic acid

2-Methylbenzo[h]quinoline

Uridine-2-13C-1,3-15N2

2,3,4,6-Tetraiodobenzoic acid

おすすめサプライヤー