Focus-independent particle size measurement from streak images: a comparison of multivariate methods

文献情報

出版日 2015-01-05

DOI 10.1039/C4AN01670J

インパクトファクター 4.616

著者

Shawna K. Tazik, Megan R. Pearl, Cameron M. Rekully, Nicholas S. Viole, Stephanie A. DeJong, Timothy J. Shaw, Tammi L. Richardson, Michael L. Myrick

原文を見る

要旨

Our laboratories have recently developed a flow-through imaging photometer to characterize and classify fluorescent particles between 3 and 47 μm in size. The wide aperture of the objective lens (0.7 NA) required for measuring spectral fluorescence of single particles restricts the depth of field, such that a large sample volume results in many particles that are out of focus. Here, we describe numerical methods for determining the size of these objects, regardless of their distance from the focal plane, using image processing and multivariate calibration. An intensity profile is extracted from the images and is used as the input for a variety of calibration methods, including partial least squares, neural networks, and support vector machines. The capabilities of these methods are examined to establish the best method for particle sizing that is independent of focus. We found that support vector machines provided the best results, with size estimation error of ±3.1 μm.

掲載誌

Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Focus-independent particle size measurement from streak images: a comparison of multivariate methods

文献情報

おすすめジャーナル

関連文献

Composition dependence of X-ray stability and degradation mechanisms at lead halide perovskite single crystal surfaces

From brew to clean fuel: harnessing distillery wastewater for electrolysis H2 generation using nano scale nickle selenide water oxidation catalysts

Carbon doped hexagonal boron nitride as an efficient metal-free catalyst for NO capture and reduction

Energy transfer from two luteins to chlorophylls in light-harvesting complex II study by using exciton models with phase correction

Trapping and thermal migration of the first- and second-row atoms in Ar, Kr and Xe crystals

Dendrite-free deposition and side-reaction suppression of zinc anodes achieved via constructing synergistic interface buffer layers

Reinforcement learning-based control for the thermal management of the battery and occupant compartments of electric vehicles

Sufficient driving force for quinoidal isoindigo-based diradicaloids with tunable diradical characters

Switchable asymmetric transmission with broadband polarization conversion in vanadium dioxide-assisted terahertz metamaterials

Combining experiment and energy landscapes to explore anaerobic heme breakdown in multifunctional hemoproteins

こちらもおすすめ

2-メトキシ-4-(メチルスルフィニル)アミンの主な用途は何ですか？

4-溴甲基-3-甲氧基苯甲酸は安全ですか？

4,6-二氯-N-甲基ピラミジンアミンの代替品はありますか？

6-氯-4-甲基-1H-吲哚を含む廃棄物はどのように処理すべきですか？

2-フローユロ-4-(トリフルオロメチル)ベンゾイドについて「に適用される法規ガイドラインは何ですか」

4-ニトロフェニルN-[(ベンゼルオキシルカーボンイル]グリシングリシングリシン酸はどのように保存すればよいですか？

イソデスロラタドリンの代替品はありますか？

5-甲氧基-1,2,3,4-四氢异喹啉盐酸盐はどのように合成されますか？

4-アミノ-5-メトキシ-2-トルエンサルホニック酸についての法規ガイドラインは何ですか？

甲基孕酮を取り扱う際の実験室安全事項は何ですか？

掲載誌

Analyst

おすすめ化合物

3,5-Dichloro-4-hydrazinopyridine

9-(1,1':4',1''-Terphenyl-4-yl)-9H-carbazole

6-Chloro-2-iodo-3-(trifluoromethyl)pyridine

dimethyl 1,1'-ferrocenedicarboxylate

2-Methylbenzo[h]quinoline

おすすめサプライヤー