Label free detection of specific protein binding using a microwave sensor

文献情報

出版日 2014-08-04

DOI 10.1039/C4AN00909F

インパクトファクター 4.616

著者

Marcela Salazar-Alvarez, Olga Korostynska, Alex Mason, Ahmed Al-Shamma'a, Jakki C. Cooney, Edmond Magner, Syed A. M. Tofail

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要旨

The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

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Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Label free detection of specific protein binding using a microwave sensor

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