Covalently incorporated protein–nanogels using AGET ATRP in an inverse miniemulsion

文献情報

出版日 2011-03-24

DOI 10.1039/C1PY00050K

インパクトファクター 5.582

著者

Saadyah E. Averick, Andrew J. D. Magenau, Antonina Simakova, Bradley F. Woodman, Andrew Seong, Ryan A. Mehl, Krzysztof Matyjaszewski

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要旨

Using a genetically engineered protein, containing a non-natural amino acid bearing an atom transfer radical polymerization (ATRP) initiator, protein–nanogel hybrids (PNHs) were synthesized by activator generated by electron transfer (AGET) ATRP in an inverse miniemulsion. The route presented is an appropriate synthetic strategy to covalently and site specifically incorporate green fluorescent protein (GFP) into well-defined nanogels. These PNHs were analyzed using dynamic light scattering (DLS), UV-visible fluorescence spectroscopy and confocal microscopy to confirm the successful integration of GFP proteins into each nanogel (NG), while preserving its native tertiary structure.

掲載誌

Polymer Chemistry

CiteScore: 8.6

自己引用率: 7.3%

年間論文数: 457

Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.

Covalently incorporated protein–nanogels using AGET ATRP in an inverse miniemulsion

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