Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy

文献情報

出版日 2010-02-23

DOI 10.1039/B919729J

インパクトファクター 3.676

著者

R.-P. Baumann, M. Schranz

原文を見る

要旨

The first AFM images of strongly bent purple membranes as well as the first single molecule force spectra of bacteriorhodopsins embedded therein are presented. AFM images of purple membranes to date always showed a flat membrane topology. Bacteriorhodopsin variants like BR-D85N and BR-D85T resemble an intermediate state of wild-type BR which is slightly ‘wedge’-shaped. Due to the strong interaction within the 2-D crystalline lattice of the purple membrane, the geometrical anisotropy of the individual bacteriorhodopsins adds up to a macroscopic change in the geometry of the purple membranes. Instead of being flat they appear like domes at physiological conditions. Single molecule force spectroscopy was employed to determine the absolute sign of the membrane curvature. As the bacteriorhodopsins in the center of the dome-like purple membranes are not supported by any solid state substrate, the presented force spectra are the first of non-supported bacteriorhodopsin, resembling the natural occurrence in the halobacterial cell.

掲載誌

Physical Chemistry Chemical Physics

CiteScore: 5.5

自己引用率: 10.3%

年間論文数: 3036

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