Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

文献情報

出版日 2008-07-01

DOI 10.1039/B804825H

インパクトファクター 4.616

著者

Maël Manesse, Valerie Stambouli, Rabah Boukherroub

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要旨

The use of Au/SiOx interfaces for the investigation of DNA hybridization using electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) simultaneously is demonstrated. Standard glass chemistry was used to link single-stranded DNA (ss-DNA) on aldehyde-terminated Au/SiOx interfaces. The layer thickness and amount of grafted oligonucleotides (ODNs) were calculated from SPR on the basis of a multilayer system of glass/Ti/Au/SiOx/grafted molecule. Capacitance and resistance values of the modified interface before and after hybridization were calculated from EIS data using an equivalent circuit and allowed the affinity rate constant, KA = 4.07 × 105 M−1, to be determined. The EIS results were comparable to those obtained by SPR hybridization kinetics recorded in parallel.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

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Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

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