Measuring complexation by single-molecule fluorescence anisotropy

文献情報

出版日 2008-04-02

DOI 10.1039/B800110C

インパクトファクター 4.616

著者

Sean M. Burrows, Dimitri Pappas

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要旨

The complexation of a fluorescent probe by a target protein was observed by single-molecule fluorescence anisotropy. Free and bound states, heterogeneities, and rare binding events can all be observed by this approach. Fluorophore-conjugated biotin was used to bind to NeutrAvidin as a proof-of-concept case. Molecular interactions were observed that could not be elucidated with conventional (ensemble) measurements.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

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Measuring complexation by single-molecule fluorescence anisotropy

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