Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies

文献情報

出版日 2007-06-05

DOI 10.1039/B701160A

インパクトファクター 4.616

著者

Ghinwa Naja, Pierre Bouvrette, Sabahudin Hrapovic, John H. T. Luong

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要旨

Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies

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