Multidimensional HRMASNMR: a platform for in vivo studies using intact bacterial cells

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出版日 2006-06-12

DOI 10.1039/B605110C

インパクトファクター 4.616

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要旨

In vivo analysis in whole cell bacteria, especially the native tertiary structures of the bacterial cell wall, remains an unconquered frontier. The current understanding of bacterial cell wall structures has been based on destructive analysis of individual components. These in vitro results may not faithfully reflect the native structural and conformational information. Multidimensional High Resolution Magic Angle Spinning NMR (HRMAS NMR) has evolved to be a powerful technique in a variety of in vivo studies, including live bacterial cells. Existing studies of HRMAS NMR in bacteria, technical consideration of its successful application, and current limitations in studying true human pathogens are briefly reviewed in this report.

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CiteScore: 7.8

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Multidimensional HRMASNMR: a platform for in vivo studies using intact bacterial cells

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