Synthesis of a caged glutamate for efficient one- and two-photon photorelease on living cells
文献情報
Olesya D. Fedoryak, Jai-Yoon Sul, Philip G. Haydon, Graham C. R. Ellis-Davies
We have synthesized a biologically inert, photosensitive derivative of the major excitatory amino acid, L-glutamate (which we call MDNI-glu) that makes more efficient use of incident light than all other caged glutamates. Laser flash photolysis of MDNI-glu in acutely isolated hippocampal brain slices evoked a rapid increase in intracellular Ca2+ concentration in astrocytes.
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