Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

文献情報

出版日 2004-12-07

DOI 10.1039/B413024C

インパクトファクター 4.616

著者

Jeffrey N. Stuart, Nathan G. Hatcher, Xin Zhang, Rhanor Gillette, Jonathan V. Sweedler

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要旨

One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

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