Thermal stability and internal dynamics of lysozyme as affected by hydration

文献情報

出版日 2004-05-11

DOI 10.1039/B402003K

インパクトファクター 3.676

著者

Stefania Cinelli, Alessio De Francesco, Giuseppe Onori, Alessandro Paciaroni

原文を見る

要旨

A differential scanning micro-calorimetric (DSC) investigation has been performed to study the thermal stability of lysozyme solvated in glycerol as a function of the water content h (grams of water/grams of lysozyme). The unfolding process is strongly dependent on h, as it is witnessed by the behavior of the melting temperature Tm which shows a significant drop in the hydration range from 0 h to 0.4/0.5 h. The specific heat difference ΔCp between denatured and native state also displays an important variation for water amounts lower than ca. 0.5 h. For higher water amounts, both Tm and ΔCp seem to attain constant values. Such hydration dependent behavior is reminiscent of the trend exhibited by some quantities characterizing the protein internal dynamics on the pico- and nano-second timescale, as estimated by elastic neutron scattering. Also the protein mobility, which is measured through mean square displacements (MSD), increases mainly for water amounts lower than ca. 0.5 h, in the same range where the protein molecular rigidity rapidly drops down. This behaviour emphasizes the crucial role played by hydration water in determining both the protein thermodynamic and dynamic quantities.

掲載誌

Physical Chemistry Chemical Physics

CiteScore: 5.5

自己引用率: 10.3%

年間論文数: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.