Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements
文献情報
Lori Beach, Claude Schweitzer, J. C. Scaiano
We report a simple and rapid method for quantitation of single-to-double stranded (ss ∶ ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss ∶ ds DNA ratio.
関連文献
Comment on “Multiconfigurational perturbation theory can predict a false ground state” by C. Camacho, R. Cimiraglia and H. A. Witek, Phys. Chem. Chem. Phys., 2010, 12, 5058
Juan Soto, Francisco J. Avila, Juan C. Otero, Juan F. Arenas
DOI: 10.1039/C0CP01917H
Pathway analysis of super-exchange electronic couplings in electron transfer reactions using a multi-configuration self-consistent field method
Hirotaka Nishioka, Koji Ando
DOI: 10.1039/C0CP01051K
Infrared photodissociation spectroscopy of H+(H2O)6·Mm (M = Ne, Ar, Kr, Xe, H2, N2, and CH4): messenger-dependent balance between H3O+ and H5O2+ core isomers
Kenta Mizuse, Asuka Fujii
DOI: 10.1039/C1CP20207C
Solvation studies of a zinc finger protein in hydrated ionic liquids
DOI: 10.1039/C0CP02487B
An in situSTM/AFM and impedance spectroscopy study of the extremely pure 1-butyl-1-methylpyrrolidinium tris(pentafluoroethyl)trifluorophosphate/Au(111) interface: potential dependent solvation layers and the herringbone reconstruction
Rob Atkin, Marcel Drüschler, Robert Hayes, Benedikt Huber, Bernhard Roling
DOI: 10.1039/C0CP02846K
Stability and growth behavior of transition metal nanoparticles in ionic liquids prepared by thermal evaporation: how stable are they really?
Kai Richter, Alexander Birkner, Anja-Verena Mudring
DOI: 10.1039/C0CP02623A
A model for the Heyrovsky reaction as the second step in hydrogen evolution
P. Hindelang, W. Schmickler
DOI: 10.1039/C0CP02748K
New electron correlation theories for transition metal chemistry
Konrad H. Marti, Markus Reiher
DOI: 10.1039/C0CP01883J
On the nature of gallium species in gallium-modified mordenite and MFI zeolites. A comparative DRIFT study of carbon monoxide adsorption and hydrogen dissociation
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Organic & Biomolecular Chemistry

Organic & Biomolecular Chemistry (OBC) publishes original and high impact research and reviews in organic chemistry. We welcome research that shows new or significantly improved protocols or methodologies in total synthesis, synthetic methodology or physical and theoretical organic chemistry as well as research that shows a significant advance in the organic chemistry or molecular design aspects of chemical biology, catalysis, supramolecular and macromolecular chemistry, theoretical chemistry, mechanism-oriented physical organic chemistry, medicinal chemistry or natural products. Articles published in the journal should report new work which makes a highly-significant impact in the field. Routine and incremental work is generally not suitable for publication in the journal. More details about key areas of our scope are below. In all cases authors should include in their article clear rationale for why their research has been carried out.











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