A comprehensive review of Dynamic Chemical Labelling on Luminex xMAP technology: a journey towards Drug-Induced Liver Injury testing

文献情報

出版日 2023-11-03

DOI 10.1039/D3AY01481A

インパクトファクター 2.896

著者

Antonio Marín-Romero, Salvatore Pernagallo

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要旨

Drug-Induced Liver Injury (DILI) is a grave global adverse event that can result in fatal consequences, causing drug failures, market withdrawals, and regulatory warnings, leading to substantial financial losses. The early detection of DILI remains a significant challenge in global healthcare. Although circulating microRNAs (miRs) show promise as clinical biomarkers for DILI, the current analytical methods for their measurement are insufficient. There is a pressing need for rapid and reliable miR detection methods that eliminate the need for nucleic acid extraction and PCR-based amplification. This review highlights recent advancements achieved by integrating Dynamic Chemical Labelling (DCL) with Luminex xMAP technology. This powerful combination has resulted in groundbreaking bead-based assays that allow (1) the direct, multiplex detection of miRs, and (2) the simultaneous testing of miR and protein biomarkers. This triple capability enables a comprehensive assessment that significantly enhances the detection and analysis of crucial biomarkers, thus improving the understanding and diagnosis of DILI. In conclusion, this review offers valuable insights into the capabilities and potential applications of these groundbreaking assays in DILI research, as well as their potential use in other diagnostic and research domains that require direct or multiplex analysis of miRs or analysis of miRs in combination with proteins.

掲載誌

Analytical Methods

CiteScore: 5.1

自己引用率: 3.7%

年間論文数: 655

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development