PCR free multiple ligase reactions and probe cleavages for the SNP detection of KRAS mutation with attomole sensitivity

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出版日 2016-10-19

DOI 10.1039/C6AN00909C

インパクトファクター 4.616

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要旨

A method to produce multiple ligated primers without PCR for a target DNA containing a single point mutation is presented. A strand displacing hairpin was introduced into the reaction, enabling separation of the ligated primer target DNA duplex without any thermal denaturing process. The ligated product was cycled to allow cleavage of fluorescence labeled substrates for RNase H on gold nanoparticles, leading to target specific fluorescence amplification. As a result, 10 attomoles of target DNA containing a point mutation in the KRAS gene were detected.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

PCR free multiple ligase reactions and probe cleavages for the SNP detection of KRAS mutation with attomole sensitivity

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