Triplex DNA logic gate based upon switching on/off their structure by Ag+/cysteine

文献情報

出版日 2015-08-25

DOI 10.1039/C5AN01371B

インパクトファクター 4.616

著者

Houya Zhu, Aiping Xin, Yubin Li, Liansheng Ling

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要旨

The formation of intramolecular triplex DNA can be regulated by Ag+ and Cys (cysteine), which switch off/on the fluorescence of the oligonucleotides, 5′-TAMRA-TTC TCT TCC TCT TCC TTC TGA CGA CAG TTG ACT CTT CCT TCT CCT TCT CTT-BHQ-2-3′ (Oligo 1) and 3′-GAA GGA AGA GGA AGA GAA-5′ (Oligo 2). Based on this principle, sensors for Ag+ and Cys are developed. The sensor for Ag+ has a linear range of 2.5 nM–40 nM and a detection limit of 1.8 nM, whereas the sensor for Cys has a linear range of 10.0 nM–120.0 nM and a detection limit of 8.2 nM. Furthermore, the fluorescence is reversible with the alternate addition of Ag+ and Cys. We constructed a DNA logic gate using Ag+ and Cys as the input, and the fluorescence intensity as the output. The DNA logic gate is simple; moreover, it has a fast response and good reversibility.

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CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Triplex DNA logic gate based upon switching on/off their structure by Ag+/cysteine

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