On-line visualization of multicolor chemical images with stimulated Raman scattering spectral microscopy

文献情報

出版日 2015-03-30

DOI 10.1039/C5AN00335K

インパクトファクター 4.616

著者

Yoichi Otsuka, Koji Makara, Shuya Satoh, Hiroyuki Hashimoto, Yasuyuki Ozeki

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要旨

We demonstrate multicolor, on-line visualization in label-free biomedical microscopy based on stimulated Raman scattering (SRS). Fast data acquisition of SRS spectral images and subsequent image generation are achieved. The loading vectors for the blind separation of chemical components are predetermined by multivariate analysis at a certain field of view (FOV) and are applied to execute on-line visualization of chemical images at other FOVs. We also show that the response time can be shortened by reducing the number of spectral data points. This on-line visualization system is expected to increase the usability of the Raman imaging system and the analytical throughput for screening.

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Analyst

CiteScore: 7.8

自己引用率: 5.6%

年間論文数: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

On-line visualization of multicolor chemical images with stimulated Raman scattering spectral microscopy

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