Direct electrochemical characterization of the interaction between haemoglobin and nitric oxide
文献情報
Chunhai Fan, Xiaocheng Chen, Genxi Li, Jianqin Zhu, Dexu Zhu, Hugo Scheer
Direct electrochemistry was carried out to investigate the interaction between haemoglobin (Hb) and nitric oxide (NO), employing a Hb–DNA film modified electrode. DNA was used as an electrochemical “promoter” for the direct electron transfer of Hb at the electrode surface. Aside from a pair of peaks corresponding to the redox reaction of Hb, two additional peaks were observed in a solution containing NO. The pre-peak, located at −0.070 V s. SCE, was proposed to come from the chemical redox reaction, i.e., NO-induced oxidation of Hb in the film, coupled electron transfer process of Hb. The other peak, at −0.684 V s. SCE, was assigned to the reduction of heme-ligated NO. These phenomena were proposed to be related to the electron transfer and binding process between Hb and NO.
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